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Wired bipolar EMG electrodes connected to a preamplifier (Digitimer D360, Digitimer Ltd, UK) were connected to a Hum Bug for removal of 60 Hz line noise before amplification (Hum Bug, Quest Scientific, India) and digitization (Qualysis Analog <t>Interface</t> <t>16</t> Channels, Qualisys, Sweden). Custom-built software (Python, v3.10.4) was used to record and display real-time EMG signals as well as segmented responses based on the TTL sync pulse from the constant current biphasic stimulator (Digitimer DS8R, Digitimer Ltd, UK). The software interface controlled a trigger pulse that was sent from the DAQ (USB-6001, National Instruments, USA) to the Raspberry Pi microcontroller which would in turn send a trigger to the stimulator (single trigger pulses in conventional waveforms, or multiple trigger pulses 100 µs apart in KHF waveforms). In addition, the DAQ sent a voltage signal to the stimulator to control the required stimulation current. The stimulator cathode (-) was connected to the popliteal fossa via a 3.2 cm diameter circular surface electrode and the 5 cm x 9 cm anode (+) was connected to the lower patella. The pulse polarity had a positive phase followed by a negative phase.
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Wired bipolar EMG electrodes connected to a preamplifier (Digitimer D360, Digitimer Ltd, UK) were connected to a Hum Bug for removal of 60 Hz line noise before amplification (Hum Bug, Quest Scientific, India) and digitization (Qualysis Analog Interface 16 Channels, Qualisys, Sweden). Custom-built software (Python, v3.10.4) was used to record and display real-time EMG signals as well as segmented responses based on the TTL sync pulse from the constant current biphasic stimulator (Digitimer DS8R, Digitimer Ltd, UK). The software interface controlled a trigger pulse that was sent from the DAQ (USB-6001, National Instruments, USA) to the Raspberry Pi microcontroller which would in turn send a trigger to the stimulator (single trigger pulses in conventional waveforms, or multiple trigger pulses 100 µs apart in KHF waveforms). In addition, the DAQ sent a voltage signal to the stimulator to control the required stimulation current. The stimulator cathode (-) was connected to the popliteal fossa via a 3.2 cm diameter circular surface electrode and the 5 cm x 9 cm anode (+) was connected to the lower patella. The pulse polarity had a positive phase followed by a negative phase.

Journal: bioRxiv

Article Title: FUNDAMENTAL LIMITATIONS OF KILOHERTZ-FREQUENCY CARRIERS IN AFFERENT FIBER RECRUITMENT WITH TRANSCUTANEOUS SPINAL CORD STIMULATION

doi: 10.1101/2024.07.26.603982

Figure Lengend Snippet: Wired bipolar EMG electrodes connected to a preamplifier (Digitimer D360, Digitimer Ltd, UK) were connected to a Hum Bug for removal of 60 Hz line noise before amplification (Hum Bug, Quest Scientific, India) and digitization (Qualysis Analog Interface 16 Channels, Qualisys, Sweden). Custom-built software (Python, v3.10.4) was used to record and display real-time EMG signals as well as segmented responses based on the TTL sync pulse from the constant current biphasic stimulator (Digitimer DS8R, Digitimer Ltd, UK). The software interface controlled a trigger pulse that was sent from the DAQ (USB-6001, National Instruments, USA) to the Raspberry Pi microcontroller which would in turn send a trigger to the stimulator (single trigger pulses in conventional waveforms, or multiple trigger pulses 100 µs apart in KHF waveforms). In addition, the DAQ sent a voltage signal to the stimulator to control the required stimulation current. The stimulator cathode (-) was connected to the popliteal fossa via a 3.2 cm diameter circular surface electrode and the 5 cm x 9 cm anode (+) was connected to the lower patella. The pulse polarity had a positive phase followed by a negative phase.

Article Snippet: The filtered signals were digitized at 50 kHz using an analog-to-digital converter (Qualysis Analog Interface 16 Channels, Qualisys, Sweden) and then sent to a host computer running Qualisys Track Manager (v2021.1, build 6350).

Techniques: Amplification, Software, Control